The production of doubled haploids DH has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes.
This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for may crops including some woody species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations.
Requests from researchers and trainees for systematised protocols dealing with the production of doubled haploids in various species were instrumental in initiating work on this Manual, and the idea to prepare it was strongly supported by European scientists working under the EU large scale program 'COST ' on 'Gametic cells and molecular breeding for crop improvement'. Many of the authors who provided protocols for the Manual participated in these activities, thereby helping to transfer DH technology in countries and over continents. Interestingly, these results are in line with those from Lippmann et al.
In this crosstalk, hormones play a key role.
Besides the role described for the principal hormones, either when they act endogenously or when applied exogenously, this review presents new and interesting notions about their involvement in this process. A remarkable example of the application of this kind of knowledge is the study brought by Chiancone et al.
Together, the papers of this Research Topic show some relevant advances in the understanding of the processes that lead to the formation of DH plants, and in their application to improve its performance in recalcitrant genotypes. The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Bohanec, B. Google Scholar. Dunwell, J. Haploids in flowering plants: origins and exploitation. Plant Biotechnol. Dwivedi, S. Haploids: Constraints and opportunities in plant breeding. Forster, B. The resurgence of haploids in higher plants.
Trends Plant Sci. PubMed Abstract Google Scholar. Guha, S.
In vitro production of embryos from anthers of Datura. Nature , Karimi-Ashtiyani, R.
Point mutation impairs centromeric CENH3 loading and induces haploid plants. Natl Acad. Already have an account? Login in here.
Haploid plants have the gametophytic number of chromosomes. The conventional methods employed by plant breeders for their production are cumbersome, time-consuming, laborious and rather inefficient. However, with the introduction of in vitro techniques, especially anther culture. Doubled haploid technology is an important tool for plant breeding. It allows for significant time reduction in the achievement of homozygous breeding lines of.
Plant Biotechnology. Journal home Advance online publication Journal issue About the journal. Original Papers. Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR Method optimization and molecular characterization.
Keywords: anther culture , doubled haploid , rice hybrid , segregation distortion , SSR. Browse "Advance Publication" version.